"Mbappe is still suffering from inflammation in his right ankle, which requires further examination. He will undergo tests today in Madrid," the FFF said in a statement.
The 26-year-old forward scored two goals and delivered one assist during the 4-0 victory over Ukraine on Thursday, when France clinched their spot at the 2026 World Cup.
Manu Kone and Eduardo Camavinga will also miss the game. The Roma player is suspended after being booked against Ukraine, while the Real Madrid midfielder, who did not feature on Thursday, has a "muscle strain in his left hamstring."