Tuchel is required to only name 23 players for a matchday team, with Ezri Konsa and Djed Spence returning after recovering from injury alongside Myles Lewis-Skelly, who is back after a suspension.
Konsa had missed the 2-1 win against Croatia on Thursday due to a knee injury, which is being managed by the England medical staff, while Spence was absent due to a muscle problem.
Their return is a major bonus for England, with Arsenal star Konsa expected to start at the Estadio Metropolitano.
However, as per UEFA's requirements, Jarred Branthwaite, James Garner and Josh King will all reportedly miss out in the Spanish capital. All three are still expected to travel and be there with the team.
England need to win in Madrid to claim top spot in the group, but both sides have already qualified for the 2027 quarter-finals.
